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Since a constitutive promoter drives exogenous GATA3 expression, this experiment validates, on the one hand, the MPA-mediated post-translational downregulation of GATA3 and, on the other hand, demonstrates the requirement of phosphorylation at serine 308 for increased GATA3 protein turnover.
This in essence, validates on a large scale the notion that ET analysis identifies key functional residues consistent with the conclusions of many experimental case studies [36].
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The minimized signatures were built on the training dataset and be validated on the validation dataset.
Atlas validation: The template was validated on 16 subjects.
The best model was also validated on 10 fold cross validation.
The test was validated on samples previously determined to contain one or none of these mutations.
Models were cross-validated on independent data points using a leave-one-out jackknife method.
Subsequently the models were validated on site.
The method was validated on simulated data.
Validated on a sample of 729 children.
Growth hackers are validated on a post-hoc basis.
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