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The results were validated using a standard leave-one-out cross-validation approach.
The total extraction and analysis protocol was validated using a standard reference material as well as by spiking experiments.
Finally, the analytical method was validated using a standard mixture of nine representative compounds.
Confirmation of reperfusion was validated using a standard gadolinium-based method.
All primers were validated using a standard curve of five serial dilutions so that all primer efficiencies were between 95 105% (Tables 1 and 2).
The methodology employed to quantify PAHs in soil extracts was validated using a standard reference material (SRM 2260, National Institute of Standards and Technology, Gaithersburg, MD).
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A previously developed search filter for use on OVID Medline validated using a gold standard set of references identified through hand searching, achieved an unacceptably low sensitivity (45.4%).
Furthermore, the accuracy of our TCSPC system was validated using a NIST certified Fluorescein standard (Invitrogen) at pH 10, and yielded a lifetime value of 4.08±0.00, (mean±SD, n = 3; Fig. 1A).
The system was trained using data from 623 clinical encounters and validated using a clinical expert as a gold standard against a mutually exclusive set of 901 records.
The performance of the two-dimensional classifier was validated using an independent test set of standard samples from the remaining 50 patients.
Primer efficiencies were originally validated using the standard curve method with all subsequent experiments using a 1∶50 dilution of cDNA and results analyzed using the comparative Ct method.
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