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Promising candidates can then be validated using a method with higher accuracy and proven reliability, such as ELISA.
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A unique quantitative high-performance liquid chromatography mass spectrometry (HPLC MS) method to investigate the energy state in cells and tissues was developed and validated using a chromatographic method designed to (i) separate and quantify more than 11 nucleotides without the use of phosphate buffer and (ii) minimize the potential ion suppression common to other nucleotide methods.
Once a prediction model is developed from a data set, it should be validated using a resampling method or an independent data set.
A set of genes altered in microarray experiments were validated using a second method like semi quantitative RT-PCR.
Final prediction models were validated using a bootstrap method for 1,000 replicates (Stat version 9; StataCorp LP, College Station, TX, USA).
The developed theory has been validated using a novel experimental method.
The correlation between FAA and skid-resistance is validated using a Forward Selection method.
A kinetic model of TMAH degradation, including cryptic growth of ordinary acidogens, acetotrophic and hydrogenotrophic methanogens from the decayed TMAH-degraders was built, and validated using a quantitative PCR method.
The Norwegian version has not previous been validated using a mixed distribution method.
Additional mutations were found with this procedure and the results obtained by ddPCR were validated using a deep sequencing method.
The OPLS-DA models were validated using a sevenfold cross-validation method, and the quality of the model was described by the parameters RX and Q values.
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CEO of Professional Science Editing for Scientists @ prosciediting.com