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ERK1/2 was chosen for these experiments since it was consistently upregulated at 5 min within most cell types, and there is no commercially available, flow cytometry validated total MK2 antibody.
In order to test the predictions for increased cholesterol efflux and decreased lipid oxidation resulting from the IPA analysis described above, we validated total cholesterol and HDL levels in a separate and larger cohort (n = 104) of PFO closure patient samples (Table 1) using a standard commercial colorimetric assay.
Each patient completed a self-administered, validated Total Knee Function Questionnaire as well as the SF-36.
Hence, validated total cut-off scores on the MoCA and IHDS were not used as the sole criterion of cognitive impairment in the application of HAND criteria.
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The OPD register line listings were used to validate aggregate disease totals for 1 month per year from 2004 2009 for the previous indicators.
FLUSH was calibrated against total runoff and drain discharge data from 2010 to 2011 and validated against total runoff in 2012.
The model was validated using total transmitted radiation (TTR) measurements.
The method was also validated using total error concept.
The physically based fatigue lifetime calculation approach "PhyBaLSIT" (SIT = strain increase test) was applied and validated for total strain controlled fatigue tests.
When comparing the results of sets 1 and our sequenced miRNA/target, we validated a total of 27 conserved interactions between small RNAs and corresponding transcript genes.
However, this variability may, at least partially, be explained by the inconsistency in methodologies used as none of surveys used a dietary assessment tool validated for total water intake or beverage and drink intake.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com