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The repression of the Pttg1 locus at the transcriptional level was also confirmed at the level of protein expression by means of WB assays which also confirmed and validated the patterns of microarray-based differential expression identified in pancreatic islets for several other randomly selected, repressed or induced loci listed in Table S1 (Additional file 1).
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Large-scale surveys have validated the pattern of increasing expenses/consumption of childrearing.
To examine and potentially validate the pattern of COX5A upregulation in humans reported in Uddin et al. [ 24], a SYBR green-based quantitative real-time quantitative PCR (qRT-PCR) strategy was designed to test the same individuals and species originally investigated by the microarray experiments: Homo sapiens (N = 3), Pan troglodytes (N = 1), Gorilla gorilla (N = 1) and Macaca mulatta (N = 3).
Furthermore, RT-qPCR validated the expression patterns of eleven of these differentially expressed miRNAs.
We validated the expression patterns of a number of genes through a different method, k-PCR, to rule out possible artifacts of microarray analysis and to provide additional confidence that these transcripts are in fact differentially expressed in developing DGAT transgenic seeds.
GC MS analysis was carried out as supportive method to validate the patterns that stem from response of the NA-NOSE to the breath samples of the HNC and LC patients, and healthy controls.
qRT-PCR analysis further validated the expression patterns of some significant genes.
Twenty-three meaningenesgenes were found, and quantitative RT-PCR analysis validated the expression patterns of 12 significant genes.
To address these questions, we identified and validated the major patterns of response to RT-CGM, using data from the largest clinical trial to date of RT-CGM versus SMBG alone in people with type 2 diabetes not taking prandial insulin.
Real time PCR of 20 candidate genes validated the expression pattern of some genes in symptomatic and asymptomatic leaves infected with CaLam or CaLas.
In our studies, we validated the expression pattern of cancer-testis antigens in resected specimens of pancreatic cancer and tested the hypothesis that treatment of pancreatic cancer cells with chromatin remodeling agents would render them more sensitive to antigen-specific T lymphocytes.
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