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To determine whether the changes in miRNA expression observed with age are consistent in a larger number of individuals, we validated the levels of a number of miRNAs in 14 young and 14 old participants (see Table 1B for demographics).
We validated the levels of KIAA1199 in tumors using immunohistochemistry. MDA-MB-231-ShNC tumors showed intense KIAA1199 expression whereas MDA-MB-231-ShB tumors showed very little or no immunostaining for KIAA1199.
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Although the visual detection of regional wall motion abnormalities is defined as a basic competence in critical care echocardiography, no previous study in ICU setting has validated the level of training required for its acquisition.
We also performed single-cell cloning and validated the level of cyclin E protein expression in these subcloned ED-1 cells.
To validate the levels of gene expression found by microarray analysis, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) experiments were performed with formalin-fixed, paraffin-embedded (FFPE) tissues from an independent EAC patient group (N = 52).
To further validate the levels of expression of these genes, q-RT-PCR was performed using sequence-specific primers.
This is an area where more data are needed to validate the levels of uNGAL, the degree of renal impairment and if this also predicts later morbidity.
We then employed immunoblotting to validate the levels of the selected genes, up- and down-regulation of which was detected by the genome-wide transcriptional profiling of the respective cells.
A major limitation of the current study is that no diagnostic interviews to assess for Major Depressive Disorder or Episode were concurrently undertaken with participants to validate the levels of depression found with the CDS and the BDI-II.
We also assessed the number of UV-induced mutants to validate the level of mutagenesis that is DinB-independent.
To validate the level of C3 and its activation, Western blotting for C3, corresponding fragment C3a and C3b were performed in the non-diabetic and diabetic serum.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com