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Ultrafiltration-based affinity mass spectrometry validated the interaction between -crebanine (1) and 5-HT2C receptor, and estimated the affinity of the ligand for the receptor (Kd ~ 0.34 μmol/L).
We validated the interaction between GST-PINIT and Ago2 using GST pulldowns.
We validated the interaction between Siglec-E and Tlr4 using both pull down and bi-directional immunoprecipitation assays.
To corroborate these findings in breast cancer, we validated the interaction between p120 and Kaiso in mILC-1 cells by co-immunoprecipitation (Fig. 2A).
As shown in Fig. 3A, IP with anti-PIM-1 antibody successfully pulled down HP1γ, while reciprocal experiment using anti-HP1γ antibody validated the interaction between PIM-1 and HP1γ in senescent cells.
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NMR binding analysis further validated the interactions between histones and selected bromodomain.
We were able to experimentally validate the interaction between 7 of the 8 links assessed (87% validation), defining a statistical association between the levels of the parent and the child node (Table 3).
The results validate the interaction between the laccase producer and lignin components of treated sample.
To validate the interaction between XB21 and LRR1, His-XB21 was co-incubated with GST or GST-LRR1 that were purified from E. coli.
To further validate the interaction between Esat6 and Hcl1, in vitro pull-down assays were performed.
Further investigation will be needed to validate the interaction between PCK1 and HLA B*4402.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com