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We validated the induction of six of the most highly over-expressed genes by qPCR.
We then validated the induction of CXCL8 and LIF in human astrocytes by FGF1 further by both qPCR and ELISA.
Even though we validated the induction of all six targets in cultured macrophages, their fold induction was lower in these cells than in the membranes.
The positive expression of Ki/67 in the islets validated the induction of islet β-cells by IL-22 (data not shown).
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Histological examinations were additionally performed to clinically validate the induction of OA.
To further validate the induction of PDGFRα and PDGFRβ following IGF-IR transgene downregulation several cell lines were utilized.
To validate the induction of autophagy upon FAC treatment, we transfected T80 cells with enhanced green fluorescent protein (EGFP LC3 plasmid followed by 250 μM FAC treatment.
In order to investigate and validate the induction of an Nrf2 response to IR, we utilized a time course post IR to examine investigate Nrf2 protein expression levels as well as mRNA expression levels of the downstream target Txnrd1 in each of the 3 WT pre-B lines.
To validate the induction of ROS by PL and to determine the role of ROS in PL-mediated autophagy, we first detected the ROS levels in PL-treated U2OS, MEF and HeLa cells through flow cytometry using the redox-sensitive fluorescent probe dichlorofluorescein diacetate.
Histologic examinations were also performed to validate the clinical induction of RA in the rats.
Histone deacetylase-1 and 2 were validated as potential targets mediating the induction of fetal haemoglobin in primary erythroid cells.
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