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Thus, the Q-PCR results validated the gene expression patterns of the genes tested.
qPCR analysis validated the gene expression profiles, indicating that expression of Wnt4 is elevated in FPSC (5-fold ) and APSC (8-fold) relative to mature cells (Sca-1Neg).
We also validated the gene expression profile of candidate genes, including AMP genes such as Diptericin B, Diptericin (Dpt), Attacin (Att), Cecropin (Cec), Drosomycin, and Metchnikowin as well as stress-related genes such as basket, eiger, and Turandot C by real time PCR analysis and we found that real time PCR results were consistent with those of the microarrays (Fig. 3C).
Real-time RT-qPCR analyses of both training and testing sets validated the gene expression profiles of MMP11 and HPSE2.
We also validated the gene expression profiles on an independent set of breast cancer surgical specimens by reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR).
Subsequent work refined and validated the gene expression signatures of subtypes (Sorlie et al, 2003; Sotiriou et al, 2003; Calza et al, 2006; Kapp et al, 2006) and developed signatures to prognosticate patient survival and predict response to therapies (van de Vijver et al, 2002; van 't Veer et al, 2002; Paik et al, 2004; Ma et al, 2006).
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The expression level of four differentially expressed genes was determined using quantitative RT-PCR to validate the gene expression data from RNA-seq (Additional file 1: Table S1 lists the primers).
To validate the gene expression profile obtained from the previous microarray analysis and to further study the biological functions of these genes in endometrial cancer.
To validate the gene expression profiles data in autotetraploid rice hybrids, twelve genes were selected for the quantitative real-time reverse transcription (qRT-PCR) analysis (Additional file 13: Table S6).
To validate the gene expression profiling data for FLJ10540, Q-RT-PCR was performed on 16-pairwise lung adenocarcinoma tumor and adjacent non-tumor samples.
To further validate the gene expression profiles, we then experimentally addressed the timing of accumulation of CD4+ T cells expressing IFNγ in M.tuberculosis-infected lungs by flow cytometry.
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