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To demonstrate the power of CSILRR for detecting biologically relevant interactions, we predicted and validated the functions of uncharacterized LRR-RKs in plant growth and immunity.
In addition to the loss-of-function assay, we also validated the functions of notch1a and notch3 through a gain-of-function approach.
RNAi analysis further validated the functions of HvZIP3 and HvZIP8 related to low grain Cd accumulation.
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We validated the function of our model by measuring the accuracy of the model for the prediction of gene knockout essentiality.
Bi et al. [ 24] developed a growth system for rice by limiting N and identified N-responsive genes, validated the function of an early nodulin gene, OsENOD93-1, by over-expressing in rice.
We further validated the function of miR-23b-3p in MDR in a xenograft tumor model and clinical GC specimens, showing that this miRNA also regulated drug resistance in vivo.
Taken together, microarray analysis validated the function of endothelial S1004A in angiogenesis.> Using B16BL6 tumor tissues little expressing S100A4, we stained tumor microvessels for CD31 and S100A4 and found that there are subpopulations of endothelial cells in tumors, S100A4-positive anegativetive ones.
57 In another work, Yang et al integrated gene expression with gene copy number alternation, DNA methylation, associated miRNA expression, and miRNA target prediction to identify key regulatory miRNA genes that regulate ovarian cancer development and then experimentally validated the function of one predicted miRNA gene.
By expressing 4-OH-ICN biosynthetic enzymes in Saccharomyces cerevisiae and Nicotiana benthamiana, we reconstitute the complete pathway in vitro and in vivo and validate the functions of its enzymes.
The test track is driven six times by expert drivers to validate the functions of ViDCo.
Future research on such targets will be informative for validating the functions of the identified miRNA in ARDS and provide more comprehensive understanding of mechanistic knowledge.
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