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Individual proteomic assays using a 96-well plate based sandwich ELISA validated the expressions of IL-6, IL-8, VEGF, TIMP1 and MCP1/CCl2.
As ERBB2 has potential for targeted therapy, we validated the expressions of ERBB2 via qPCR of 7 samples in Dataset1 that were found to be amplified or deleted.
We also validated the expressions of other miRNAs that have potential binding sites for ZEB1 predicted by TargetScan, PicTar, Miranda, and miRDB, including miR-23b, miR-199a, miR-96, and miR-150.
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We validated the expression of five novel miRNAs and found three to be differentially expressed.
We have validated the expression of different isoforms as well as novel genes in developing mouse lens by qRT-PCR.
We validated the expression of alternate isoforms of Pax6 and Cdk4 by RT-PCR and Sanger sequencing across developmental stages.
Our study further validated the expression of these two proteins using the Western blot technique.
We validated the expression of eight genes with varying degrees of evidence for involvement in ovarian cancer.
qPCR analysis validated the expression of Tcfap2c, indicating that it is significantly elevated in the UGE (11-fold) relative to the UGM (Figure 2).
Then we validated the expression of these miRNA observed in the microarray in 14 neonate mice (+1 day after bird) and 6 adult mice by qRT-PCR.
Among these, we validated the expression of Fabp5 and Acot1.
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