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Furthermore, RT-qPCR validated the expression patterns of eleven of these differentially expressed miRNAs.
We validated the expression patterns for selected genes, demonstrating an association of almost all most differentially expressed genes with proliferation or cell cycle arrest, consistent with previous senescence studies.
qRT-PCR analysis further validated the expression patterns of some significant genes.
Twenty-three meaningenesgenes were found, and quantitative RT-PCR analysis validated the expression patterns of 12 significant genes.
We validated the expression patterns of a number of genes through a different method, k-PCR, to rule out possible artifacts of microarray analysis and to provide additional confidence that these transcripts are in fact differentially expressed in developing DGAT transgenic seeds.
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In our studies, we validated the expression pattern of cancer-testis antigens in resected specimens of pancreatic cancer and tested the hypothesis that treatment of pancreatic cancer cells with chromatin remodeling agents would render them more sensitive to antigen-specific T lymphocytes.
Using reverse transcription (RT -qPCR, we validated the expRT -qPCRpattern of f11r.
Quantitative real-time PCR experiments validated the expression pattern of stigma preferential genes including homologous grass SI candidate genes.
Real time PCR of 20 candidate genes validated the expression pattern of some genes in symptomatic and asymptomatic leaves infected with CaLam or CaLas.
In this study, we have validated the expression pattern of the antiangiogenic factor METH-2 in NSCLC following the identification of the tumour under-representation of its transcript in a microarray analysis of primary disease (Heighway et al, 2002).
We used Genevestigator expression datasets (microarray based) to further validate the expression patterns of a subset of those genes expressed more highly in somatic embryos than in leaf tissues by RNA-Seq.
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