We validated the expression of five novel miRNAs and found three to be differentially expressed.
We have validated the expression of different isoforms as well as novel genes in developing mouse lens by qRT-PCR.
We validated the expression of alternate isoforms of Pax6 and Cdk4 by RT-PCR and Sanger sequencing across developmental stages.
They subsequently validated the expression of miRNAs in plasma-derived EVs and showed significantly higher levels of miR-1246 and miR-21-5p in breast cancer patients (n = 16) compared with healthy controls (n = 16), each with AUCs of 0.69 and a combined AUC of 0.73 [118].
Our study further validated the expression of these two proteins using the Western blot technique.
We validated the expression of eight genes with varying degrees of evidence for involvement in ovarian cancer.
Then we validated the expression of these miRNA observed in the microarray in 14 neonate mice (+1 day after bird) and 6 adult mice by qRT-PCR.
qPCR analysis validated the expression of Tcfap2c, indicating that it is significantly elevated in the UGE (11-fold) relative to the UGM (Figure 2).
In addition to those described here, we validated the expression of UCHL1, PTPN6, TNFR1, SELENBP1, TNFR1, TNFRSF10D, S100A4, and several MAGE genes by semi-quantitative or real-time RT-PCR, or Western blots.
Using a diverse panel of human tissues and cell lines, we validated the expression of a subset of three previously uncharacterized genes (clusters Hs.295012, Hs.518391, and Hs.559350) to be highly restricted to melanoma/melanocytes and named them RMEL1, 2 and 3, respectively.
Using miRNA-specific stem-loop qPCR assays [24] we validated the expression of ten most upregulated miRNAs from the significant list, and also prioritizing miRNAs which appeared in previous studies on pancreatic islets or insulin-secreting beta cell lines such as miR-124, miR-376a, miR-132 and miR-212.
