Homology-based structure modeling and the biochemical analysis of laboratory-generated protein variants validated the contribution of the five positively selected sites to functional divergence of OryzaTPS1.
To generalize these observations, we validated the contribution of STAMP2 to cell growth in an additional androgen-responsive PCa cell line, VCaP.
Noise was measured in >2,500 proteins using GFP-tagged yeast strains [ 3] and this validated the contribution of mRNA dynamics to protein noise.
Data from clinical trials indicating that B cell depletion with rituximab is highly therapeutic, and well tolerated, in RA patients have validated the contribution of B cells in the pathogenesis of RA [ 2].
Next, to generalize our findings, we validated the contribution of RARRES3 to lung colonization in patient-derived CN37 cells (Gomis et al, 2006) and in 4T1 mouse-spontaneous ER− metastatic BC cells (Aslakson & Miller, 1992).
We subsequently validated the contribution of increased actomyosin contraction and stress fiber instability to doxorubicin-induced cell detachment and apoptosis using pharmacologic inhibitors, blebbistatin, and cytochalasin D. Blebbistatin is a direct inhibitor of myosin II ATPase activity, and cytochalasin D inhibits actin polymerization.
The experimental results from these four bimodal knuckle databases validate the contributions of the proposed work.
Having the above thoughts in mind, we discuss the details of an evaluation we carried out to validate the contribution of the service to the query construction process.
However, we did identify a consistent association of this gene with type 2 diabetes in our Chinese population, further validating the contribution of TCF7L2 on susceptibility to the disease.
In order to validate the contribution of MEK1/2 kinases in the positive regulation of CIP2A expression, AGS cells were treated with UO126, a more specific and potent MEK1/2 inhibitor than the previously used PD98059, and CIP2A protein expression was studied 48 hours post treatment.
