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In this study, we validated the conservation of motifs and reconstructed pathways based only on known phosphosites.
We validated the conservation of tissue expression patterns of five miRNAs in human, mouse, and zebra finch by Northern blot analysis.
We validated the conservation of A. oryzae TRs in the A. flavus genome by checking for their presence in the corresponding orthologous region of the A. flavus reference strain NRRL 3357 genome.
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To validate the conservation of SSRs across the varieties and species, at least one amplicon from different genotypes/species and multiple amplicons from the same genotypes were sequenced.
To validate the conservation of this arginine and other GSH-binding residues, all Epsilon and Delta class GSTs were retrieved from complete genomes of D. melanogaster and A. gambiae for protein sequence analysis.
Similar results were observed in a parallel assay using a human TAF2 C-terminus (990 1199) fusion protein, validating the conservation of the inhibitor target sites between Drosophila and human TAF2 proteins.
In addition to these computational approaches, we assessed the similarity of expression profiles in mature leaves to experimentally validate the sequence conservation of these species using heterologous hybridization to potato cDNA microarrays.
The peptide/antibody recognition validated our system showing the conservation of peptide tridimensional structure with a positive response to specific antibodies.
To reduce the number of false positives, target sites may be validated by conservation of sites across species.
Furthermore, we validated the credibility of the identified erythroid-specific KLF enhancers through integrated bioinformatic and biochemical approaches to determine sequence conservation, TF binding, and histone modification markers.
Primer sequences and related information for 43 monomorphic EST-SSR primers are available as Appendix 2. The conservation of SSRs across species has been validated by sequencing one amplicon from each species.
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CEO of Professional Science Editing for Scientists @ prosciediting.com