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We also analyzed several pluripotency markers at the protein level by immunoblotting (Figure 2B; for a complete description of the antibodies used, see Table S3) and immunofluorescence (Figure 2C, Table S3), and validated that all ASThiPS cell lines resembled hES cells in terms of pluripotent gene expression.

In the case of the mitochondrial DUB USP30 [7], we have validated that all four oligos, which inhibit scattering, effectively suppress the expression of transfected USP30-GFP and endogenous mRNA levels (data not shown).

We have validated that all of W-patterns with the maximum support are directly associated with the functional sites of the query protein by examining locations of the discovered patterns on available protein structures.

Assessment of the propagation of pathology to different grey matter regions validated that all cases could be allocated to one of four neuropathological stages, although Stage 4 cases were found to differ significantly in age of onset (~10 years older) and disease duration (shorter duration than Stage 3 and similar to Stage 2).

Two health-related research assistants validated that all the basic content had been covered and discussed by the lecturers, using a check-list.

Twelve TFs (3 NAC TFs, 2 HD-Zip TFs, 1 bHLH TF, 1 DOF TF, 1 C2H2L TF, 1 MYB TF, 1 AP2 TF, 1 ARF TF and 1 GRAS TF) were chosen for further expression analysis in well-watered and drought-stressed ramie by qRT-PCR and the result validated that all these 12 TFs were drought stress-responsive TFs.

The first test flight took off Monday at 12 11 p.m. Pacific time from Paine Field in Everett, Wash., to validate that all systems on the plane are working as designed.

Alternatively, users may use the average of all probe sets without validating that all indeed report similar transcription rates.

In all cases, a visual check was performed to validate that all cells had been identified correctly using the software, or through manual counting [see Additional file 1].

Peak finding and de novo motif prediction on combined reads were then used to validate that all lobChIP experiments for the sequence-specific TFs had been successful.

To validate that all three isoforms did indeed associate with the blocks and that this was not a by-product of antibody cross-reactivity, we transiently transfected BAZ1B knockout cells with HP1α, HP1β, or HP1γ green fluorescent protein (GFP) expression constructs.