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Ideally, error estimates for genotype calling should be based on independently obtained and validated sequence data.
We observe a very curious distribution of the B Box motif if we consider the sequence GTT(C/T GAGAC (B'Box in Figure 2) wherein we restrict the pattern to the experimentally validated sequence.
Harismendy et al. (2009) estimated the variant accuracy of the three main NGS platforms to be on the order of 95% at high sequencing coverage, whereby variant accuracy was defined to be the proportion of ABI-Sanger validated sequence variants that are correctly called as homozygous or heterozygous variants on the basis of NGS read alignments.
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(B) qRT-PCR validated sequencing result in (A).
Quantitative RT-PCR and validated sequencing result on Erk1/2 (Fig. 7A and 7B).
The most widely used and most extensively validated sequences for T1 mapping are MOLLI-based sequences [10, 42, 47].
The addition of our eight validated sequences to the database strengthens the evidence of phylogenetic miRNA sequence conservation.
These computational models and their discoveries seem to be promising, because they were using large set of experimentally validated sequences.
We applied ESPRIT to an only partially sequenced genome of Callorhinchus milii, and validated its prediction on comparison with experimentally validated sequences from GenBank and on phylogenetic analyses.
Thus, the Purpuratus donor splice site model is able to predict splice sites not only from annotated gene sequences, but also from experimentally validated sequences.
To validate sequence results of the TATA box regions, single nucleotide polymorphism (SNP) specific primers were used in a fluorescence/gel-migration assay.
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CEO of Professional Science Editing for Scientists @ prosciediting.com