To test the hypothesis that the amount of FBPase depends on the rate of cell proliferation, we determined the specific activity, which is a precise method to validate enzyme quantity.
Isolated RNAs were eluted in nuclease free water, validated for quality and quantity using UV spectrophotometry, and stored long term at −80 °C.
We validated the libraries for quantity and quality on a 2100 Bioanalyzer using a High Sensitivity DNA chip according to the manufacturer's instructions.
Finally, the cDNA library quantity was validated using a Qubit® 2.0 Fluorometer (Invitrogen, Life Technologies), while the quality was validated using an Agilent Technologies 2100 Bioanalyzer prior to the HiSeq Illumina sequencing.
Model output quantities are validated against measurements, by self-consistency tests and through inter comparisons with other radiative transfer models.
Modulation of the expression profile by Xbp1S was validated by analyzing mRNA quantities of representative cell cycle- and differentiation-related genes using quantitative PCR (Supplementary Figure 2).
RNA quality and quantity were determined using an Experion (Bio-Rad) automated electophoresis instrument and quantity data were validated by Nandrop® ND-3300 fluoRiboGreenphometeRiboGreeneen).
Consequently, few decades have witnessed a glut of research geared towards validating the quality, quantity, protective roles as well as therapeutic effectiveness of these antioxidant in African plants against oxidative stress induced diseases and disorders.
The RNA quality and quantity was further validated with a NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, DE).
A number of controls validated the absence of significant quantities of residual genomic DNA.
Differential gene expression identified by microarray analysis was validated by determining the relative quantities of several gene transcripts from infected and uninfected mice using real time RT PCR.
