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Until these new methods have been validated, quantification of the percentages of pitted erythrocytes seems most reliable to screen for potential hyposplenic patients.
For this purpose, we first validated quantification by pyrosequencing by comparing it with 2 other methods: real-time PCR and picoliter-droplet digital PCR.
The validated quantification range was 1 2000 ng/mL.
The Unified Parkinson's Disease Rating Scale UPDRSS) is a validated quantification of Parkinson's Disease signs and symptoms.
We present herein a validated quantification of mutated BRAF in a large series of human skin melanoma samples, and demonstrate that several cases are not heterozygous.
FDG uptake in each voxel must be previously normalized to the average uptake of a reference region, since without arterial blood sampling or other validated quantification methods, the standard PET procedure does not allow true quantitative measurements of glucose consumption.
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To validate quantification of pathological bone metabolism by F-fluoride PET imaging in experimental arthritis, we performed correlation analysis of quantitative PET results with semiquantitative clinical scoring data and assessment of bone surface roughness destruction by μCT.
Such works validate quantifications of Laban concepts and show that a mid-level LMA-based representation can be obtained starting from visual descriptors.
Final libraries were validated by quantification using the Qubit 2.0 fluorometer with the HS DNA quantification reagents (Invitrogen) and using the Bioanalyzer 2100 Desktop System with the DNA1000 Kit (Agilent Technologies).
We calculated the coefficient of determination (R2) of 0.99 for both the sodium and silver adducts, demonstrating isotopic quantification successfully coupled with the silver nanoshells-assisted LDI MS. We validated the quantification method for lactose by intraday, interday, intra-batch, and batch-to-batch analysis as summarized in Table S2.
A selective stability indicating HPLC method was developed and validated for quantification of impurities (process related and degradants) and assay determination of Exemestane.
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