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Our study provides a validated panel of optimal reference genes for the identification of genes differentially expressed by qRT-PCR in caprine MSC.
A validated panel of immunohistochemical (IHC) markers have been developed to approximate the classification of these subtypes, including luminal A (ER+ and/or PR+ and HER2-), luminal B (ER+ and/or PR+ and either HER2+ or Ki-67+), non-luminal HER2+ (ER-, PR-, and HER2+), basal-like (ER-, PR-, HER2-, CK5/6+ and/or HER1+), and unclassified (ER-, PR-, HER2-, CK5/6-, and HER1-) [13], [14], [14].
A validated panel of 12 single-nucleotide polymorphisms (SNPs) associated with breast cancer were measured.
We utilized a validated panel of 12 breast cancer associated SNPs.
We employed a validated panel of genetic markers associated with breast cancer incidence, with RR estimates independent of traditional breast cancer risk factors.
In order to assess the relationship between subtype and CSC markers, we have divided tumours into molecular subtypes according to a validated panel of IHC markers and stratified all analyses by oestrogen receptor status (ER).
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We also believe that it will be an integral part of diagnosis and serial monitoring of disease states in the future where one can envision a hand-held device that could monitor expression levels of validated panels of genomic biomarkers, much like the glucose monitoring systems of today.
The concentration of cytokines in the BALF after treatment with active rCARDS or HI rCARDS toxin was determined by Luminex assay using a validated panel as described [31], [32], [33].
A clinically validated panel was used for mutational profiling [ 10- 15].
This work builds on previously published work by our group in which we discovered and validated biomarker panels of acute cardiac allograft rejection.
Reported familial relationships were validated using panels of ancestry-informative [ 30] and whole-genome [ 31] genetic marker data provided by the Center for Inherited Disease Research at Johns Hopkins University and quality checked jointly by study investigators and the Coordinating Center for the NIH Genes and Environment Initiative (GENEVA; [ 32]).
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