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The foregoing studies validated our ability to derive stable vectors that incorporate MPER within hexon HVR2.
Additionally, we validated our ability to identify tumor-reactive antibodies from our patient cultures, compared to equal amounts of non-specific IgG and culture media (Figure 1, D).
Having validated our ability to accurately sort single cells, we tested the μWGBS protocol for starting amounts of 500 , 50 20, 10, 4, and 1 cell(s), using no more than 18 PCR cycles for library amplification.
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This not only validates our ability to model dielectrophoretic forces in these traps but also gives insight into the physical behavior of particles in dielectrophoresis-based traps.
We have taken care to reduce measurement variations by validating our ability to target cells using an Aplysia neuronal population.
The comparison of results between services was of particular interest, as it would validate our ability to learn useful information from the programme and would direct strategies for improvement.
DOI: http://dx.doi.org/10.7554/eLife.03282.016 To validate our ability to measure cell cell force exchanges with sub-cellular resolution, and to determine an approximate length scale over which force and E-cadherin recruitment are coupled, we divided each cell junction into shorter sub-junctional segments of different lengths and scrambled the intensity profiles within the segments.
The results of the PCA of the microarray data showed that the three replicates for each cell type clustered closely together validating our ability to separate the cell types by dissection and revealing distinct expression profiles for the AC, AF and NP cell types.
Although these efficacy results are preliminary, this study highlights the potential for use of dendrimer drug therapies in this translationally relevant large animal model, while also validating our ability to make dendrimer drug conjugates on a large scale using two clinically approved drugs.
To validate our ability to calculate partial occupancies of calcium ions by e/Å levels in Coot, refinement of metal ion occupancy was performed in Phenix on calcium ions and gave percent occupancies of 65%and71%1% for Ca1 and Ca6, respectively, in the apoPAD2 structure.
Sample return from the surface will validate our collective ability to successfully accomplish such a roundtrip to Mars in absence of the ability for end-to-end testing on Earth.
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