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Next, we validated module 17 by examining gene expression by qRT-PCR and inferred that two putative uncharacterized proteins might be potentially related to heat stress.
Using the validated module, it is found that the best PCM's configuration is when placed in direct contact with the indoor controlled environment.
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The advantage of such an approach lies in the fact that separately validated modules, for example for DNA extraction or for real-time PCR, could then be combined.
Overall, the combined use of interactome classes, network clustering methods and additional multiomic sources allows for better characterization of the modularity map, and for an assessment of the influence of integration and disaggregation on the detected and validated modules.
If a validated final module is co-expressed in at least half of the validation sets, then it is defined as a core module.
Validated targets module of miRWalk was also used to investigate hosts experimentally verified target genes of differentially expressing miRNAs in the study (http://www.umm.uni-heidelberg.de/apps/zmf/mirwalk/) [ 64].
The module search result will only contain validated motif modules by known interacting TFs currently.
Users can also search validated motif modules in the same page by selecting the 'search modules' option.
We present here a hierarchical modular cloning system that allows the creation at will and with high efficiency of any eukaryotic multigene construct, starting from libraries of defined and validated basic modules containing regulatory and coding sequences.
After KEGG and GO annotations, we have validated our modules (Table 7) by mapping them to known complexes in MIPS [ 49].
We validated these modules by examining their connectivity density, their functional enrichment in biological process GO terms and their coverage in protein complexes.
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