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Using the training set, cutoff values for CK19 and CEA mRNA were predetermined, and these levels were then validated in the subsequent validation set.
As an example, the pioneering simulation studies on potassium channels performed by Roux and coworkers even anticipated some results that were validated in the subsequent crystallographic studies (Roux and MacKinnon, 1999; Berneche and Roux, 2001).
The coupled foot boot model will be fully validated in the subsequent works under both static and dynamic loading conditions for further studies on injuries investigation in military and sports, foot wear design and characteristics of parachute landing impact in military.
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The 11C Choline PET/CT results were validated in the light of subsequent clinical and radiological follow up (FU) of 1 to 2 year, including PSA trend after targeted therapy (such as RT on the prostatic bed), other imaging modalities (such as pelvic MR and/or TRUS) and/or local biopsies.
The strength of the approach was validated in subsequent studies showing that the suppression of gene groups induced by the loss of CD8+ lymphocytes [ 12] and natural killer [ 33] and dendritic cells [ 34] substantially decreased epithelial injury, prevented bile duct obstruction, and improved cholestasis.
Based on these data, another cutpoint to establish an equivocal range was identified to align with the 2007 ASCO/CAP guidelines for HER2 testing, prespecified and validated in two subsequent positive clinical validation studies [ 22, 23].
Methods for extraction of the drugs from hair were developed and subsequent ELISA analyses were validated in-house.
The results of this pilot study were validated in a subsequent large, prospective, multicenter trial (Transfusion Requirements in Critical Care [TRICC]) conducted by the Canadian Critical Care Trials Group [ 12].
A recent evaluation of over 1000 patients with advanced oesophagogastric cancer identified several prognostic factors affecting survival outcomes (Chau et al, 2004) (the Royal Marsden Hospital Prognostic Index), which have been validated in a subsequent data set (Chau et al, 2009b).
We hypothesized that the results of our miR-eQTL mapping might help to clarify many of the associations found in the NHGRI catalog of published genome-wide association analyses, most of which have been validated in a subsequent replication study.
It was validated in four subsequent studies with various samples of teachers and pre-service teachers.
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