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In all, the hits from our initial screen were validated in the secondary screen.
Although multiple hits were identified initially from the Chembridge Diverset™ library, none validated in the secondary screen, which combined a concentration/dilution series with tests for cell viability.
TFs that could be validated in the secondary step as AbdA-binding partners (see Figure 2 ) are indicated (dotted-red box).
Two compounds, chromomycin A3 from the NPC collection and Ro 31-8220 from the kinase inhibitor library, reduced foci in the primary screen and were validated in the secondary screen.
In males MTHFR-C677T (p = 0.0036) was significantly related to serum miR-21 expression, however the association between MSR-A66G and miR-21 expression in serum was not validated in the secondary cohort (p = 0.9851) (model r = 0.174, p = 0.0218).
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Thus, weaker hits often fail to validate in the secondary screen due to random measurement error.
The following cover classes were differentiated: tropical forest, secondary forest, agriculture, urban, and water polls, which were validated in the fields.
A number (n = 23 of 79) of QIs we constructed were not validated in our secondary analysis of the Mega QI data.
The 5-substituted hit compounds were most active and were validated in a secondary assay against KDM4A and KDM4E.
8 For example, the ABCD2 score was derived and validated in secondary care as a prognostic score, yet the UK's National Institute for Health and Clinical Excellence 3 recommends its use in all referrals of suspected TIA by general practitioners (GPs) and accident and emergency (A&E) departments despite evidence of substantial disagreement between specialist and generalist scores.
This age adjusted cut-off value was subsequently validated in secondary care patients with suspected pulmonary embolism, 16 17 and in both primary and secondary care cohorts of patients with suspected deep vein thrombosis.
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