Exact(1)
c100 of the 114 genotypes were validated from the same sites used to validate the discordant genotypes.
Similar(59)
We used RT-qPCR to validate from the same RNA samples as used for the microarray analysis the differential expression of representative candidate genes.
Strong similarity was observed between the transcriptional responses of the hydroponic cultures to the NaCl and (NaCl + CO2) stresses This observation is supported from the PCA graphs g-h in Figure 2. The similarity is validated from the PCA graph i in the same figure, which includes all timepoint transcriptional profiles in all four physiological (i.e. control, CO2, NaCl and (NaCl+CO2)) states.
For HER2, no (0) or weak (1+) membrane protein immunostaining was considered low HER2 expression (HER2–); and moderate (2+) or strong membrane protein immunostaining (3+) was considered HER2 overexpression (HER2+) based on previously validated results from the same pathology laboratory comparing IHC with HER2 gene amplification measured by fluorescent in situ hybridization methods [ 17].
Finally, the diabetic retinopathy prediction models and estimates were derived from and validated in the same study sample.
The transcriptional levels of puc and Wnt5 were analyzed using SYBR Green-based qPCR analysis with primer pairs from FlyPrimerBank, validated using the same primer analysis procedure and acceptance criteria described previously.
This FFQ was previously validated in the same geographic region from which the population of the present investigation was drawn.
We feel so blessed and humbled and also validated at the same time.
Twenty-five diffexpressedy expressed genes were randomly selected and validated with the same RNA preparations that were used to generate microarray data, and 8 out of the 25 genes were again validated with the new RNA samples from the same ovary tissues by qRT-PCR.
The first is that the rule was derived from a single-center retrospective chart review study and it was created and validated in the same population.
Finally, all edits were inferred from clean sequence reads, generated from both strands of a clone, and most were also validated by obtaining the same sequence from multiple independent cDNA clones (Table 1).
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