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The procedure was validated for specificity, linearity, accuracy and precision.
After optimization, the 8-color assay was validated for specificity, precision, linearity, limit of quantitation and robustness.
A novel species-specific 14-bp PNA probe was designed for M. immunogenum based on its 16S rRNA gene sequence and was validated for specificity, by testing against a panel of other phylogenetically closely related rapidly growing mycobacteria and representative species of gram-positive, gram-negative, and acid fast organisms.
All real-time primer sequences were validated for specificity and efficiency prior to use.
Polyclonal antibodies were raised in rabbit against 15 amino acids from the second luminal loop of FIT2 (ALEGIRQEHRSKQQC) and validated for specificity by using lysates from HEK293 cells expressing FIT2 (Fig. S4).
All antibodies used in this study were validated for specificity by western blot analysis.
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The designed genus-specific primer probe systems were validated for sensitivity, specificity and amplification in the presence of background DNA.
The MELC method was validated for its specificity, linearity, accuracy, precision, robustness and stability.
The optimized PCR assay was subsequently validated for its specificity with DNA extracted from cattle, buffalo, sheep, goat and pig.
The new design has been rigorously validated for its specificity and performance using samples isolated from mycobacteria grown under different environment conditions.
The Pi9-Pro and Pi2-LRR markers were further validated for their specificity to Pi2 or Pi9 in monogenic lines harboring blast resistance genes in the Pi2/9 locus, including IRBLz5-CA (Piz-5 = Pi2), IRBL9-W (Pi9), IRBLzt-T (Piz-t) and IRBLz-Fu (Piz) (Tsunematsu et al. 2000).
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