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All analyses performed were validated for pig plasma.
A kit designed for human serum that we validated for pig serum was used.
The assays were validated for pig plasma by demonstrating that serial dilutions of plasma were parallel to the displacement curve for the reference standards.
The cotton swab was then centrifuged (Hettich Centrifuge EBA 20, Andreas Hettich Group, Ltd., Tuttlingen, Germany) at 2400 x g at room temperature for 2 minutes and the saliva extracted was stored at −20°C before being analysed using a commercially available cortisol ELISA kit (Cortisol ELISA, IBL International, Hamburg, Germany) validated for pig saliva [ 18].
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The method has been validated for use with pig saliva [ 24].
This microarray has been validated for use in pig transcriptional profiling studies by Zhao et al. [ 66].
To the authors knowledge there is no commercially available ELISA that has been validated for quantifying cortisol in pig saliva.
The AR system presented in [ 11] is validated for liver surgery on pigs where registration with 4 fiducials is used to measure its accuracy.
The optimized PCR assay was subsequently validated for its specificity with DNA extracted from cattle, buffalo, sheep, goat and pig.
This method has been validated for a number of species including sheep, 23 dogs 24 and pigs.
"We certainly feel validated for our work last night".
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