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Zhang says the method used in his paper has been validated down 0.3%, and it may be even more sensitive.
However, the hazard posed by debris in the size range from 1 mm to 10 cm is paramount in terms of personnel and spacecraft protection; thus, there is a need for a fragmentation model validated down to characteristic sizes of 1 mm.
Although intended for children 6 years or older, it has been validated down to 3 years old with good results [ 19].
Even though intended for children 6 years or older it has been validated down to three years of age with good results [ 22].
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We also observed and validated down-regulation of Gad1 by 35 and 50 days of age.
To validate down-regulation of miR-21 by BB and to investigate the effect of exogenous IL6 on BB-modulated miRNA-21 expression in MM, RPMI-8266 and U226 cells were treated without or with 75 μM or 120 μM BB, respectively, in the absence or presence of IL6 (0.5 ng/ml).
The FEA is validated by down-scaled experiments performed previously within the HISTWIN project.
We validated knock-down of the target genes by SGS in western blot.
Real-time RT-PCR validated the down-regulation of these genes at two time points (data not shown).
Using quantitative polymerase chain reaction (qPCR), we validated the down-regulation of these 2 genes not only in HepG2 cells but also in Huh7 cells.
We have validated three down-regulated miRNAs whose FC ranged from −1.3 to −5.8 and one miRNA which was found to be up-regulated (FC = 12.8), using samples for which RNA was available following NGS.
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