To validate the differently expressed genes identified by SAGE, real-time quantitative RT-PCR analysis was performed in 20 pairs of HCC and adjacent nontumorous liver tissues.
The other seven protein spots that were validated to be differently expressed in tumour-bearing mice compared with free-fed mice, but whose expression did not differ significantly compared with that seen in pair-fed mice included N-ethylmaleimide-sensitive factor, and oxoglutarate dehydrogenase, both of which were upregulated.
Although there is no indication that our rules would perform differently in resource-poor settings, they should be validated in populations differing from Swiss HIV patients.
However, this tool to assess current depressive symptoms has not been validated and the items could potentially function differently across the three ethnic groups in this study.
Among a number of interesting proteins differently expressed, two candidate markers were validated to verify the iTRAQ method.
The proportion of pixels ranked differently between the model that validated most poorly and the model that validated best was 0.72 0.88 depending on year.
The proposed model is validated with test data and used to compare the performances of differently structured condensers using refrigerants R22 and R404A.
Nine validated risk factors, each independent but differently weighted, risk factor for fracture were identified and statistically validated in FRAXTM acronym: fracture risk assessment modeling.
The choice sets used had not been formally validated, however, it was assumed before conducting the study that individuals, with different risk factors, would perceive such factors differently and this was confirmed upon the analysis of the data.
28 The choice sets used had not been formally validated; however, it was assumed before conducting the study that individuals, with different risk factors, would perceive such factors differently and this was confirmed upon the analysis of the data.
