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Semiquantitative RT-PCR validated changes in the expression levels of some of the genes are shown in (Fig. 8).
We independently validated changes in the steady state levels of 30/44 (68%) of these transcripts from both lists by qRT-PCR (Fig. 6B; Table 1).
We validated changes in the abundance of two of these proteins by western blotting extracts from additional sciatic nerves.
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To validate changes in the EMT process, phalloidin-labeled staining (Life Technologies, NY, USA) and α-SMA staining (Sigma-Aldrich) were carried out [ 30].
We have validated changes in three loci with the most prominent changes: FBXL5, SCMH1 and CACYBP.
The use of change in gait speed to validate change in the DHI-N scale may therefore be questioned.
Logistic regressions were undertaken of baseline depressive symptoms (six-item Center for Epidemiologic Studies Depression Scale (CES-D)) with (1) completion of 1- and 4-year study assessments and (2) validated change in dietary behavior in the intervention group.
To validate changes in gene expression from the microarray analysis results, real-time PCR was carried out on 18 selected genes, chosen on the basis of their high expression rate (fold change), for their specific function related to cholesterol or steroid synthesis and cholesterol transfer, and some also chosen since their function remain unknown to this day.
Quantitative real time-polymerase chain reaction (qRT-PCR) was used to validate changes in gene expression in response to androgen of 39 (13 known; 26 novel) of the 87 total genes identified by LongSAGE.
To validate changes in intraocular pressure (IOP) after phacoemulsification with intraocular lens (IOL) implantation without pressure-lowering surgery in previously unoperated eyes of normal and glaucoma patients.
To examine and validate changes in striatal dopaminergic function and regional brain metabolism during phenocoversion of preclinical Huntington's disease (pHD).
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