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Semiquantitative RT-PCR validated changes in the expression levels of some of the genes are shown in (Fig. 8).
We independently validated changes in the steady state levels of 30/44 (68%) of these transcripts from both lists by qRT-PCR (Fig. 6B; Table 1).
We have validated changes in three loci with the most prominent changes: FBXL5, SCMH1 and CACYBP.
We validated changes in the abundance of two of these proteins by western blotting extracts from additional sciatic nerves.
We showed several examples where YLoc predicted experimentally validated changes of localization sites and known sorting signals caused by mutations.
Results from the microarray transcript analysis were further validated by RT-PCR for selected genes, and our results have validated changes observed within the microarray data.
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Logistic regressions were undertaken of baseline depressive symptoms (six-item Center for Epidemiologic Studies Depression Scale (CES-D)) with (1) completion of 1- and 4-year study assessments and (2) validated change in dietary behavior in the intervention group.
It can also be useful in validating changes to the circadian model, and as an educational tool.
We used qPCR to validate changes of RNA expression that we had observed using microarrays, and confirmed the over-expression of SCARNA17, miR-21, and miR-130a.
To validate changes observed in the activity of enzymes involved in glucosinolate metabolism at the transcript and protein level, we investigated changes in specific glucosinolates by UPLC-qTOF-MS.
To validate changes in intraocular pressure (IOP) after phacoemulsification with intraocular lens (IOL) implantation without pressure-lowering surgery in previously unoperated eyes of normal and glaucoma patients.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com