Semiquantitative RT-PCR validated changes in the expression levels of some of the genes are shown in (Fig. 8).
We independently validated changes in the steady state levels of 30/44 (68%) of these transcripts from both lists by qRT-PCR (Fig. 6B; Table 1).
We have validated changes in three loci with the most prominent changes: FBXL5, SCMH1 and CACYBP.
We validated changes in the abundance of two of these proteins by western blotting extracts from additional sciatic nerves.
We showed several examples where YLoc predicted experimentally validated changes of localization sites and known sorting signals caused by mutations.
Results from the microarray transcript analysis were further validated by RT-PCR for selected genes, and our results have validated changes observed within the microarray data.
Logistic regressions were undertaken of baseline depressive symptoms (six-item Center for Epidemiologic Studies Depression Scale (CES-D)) with (1) completion of 1- and 4-year study assessments and (2) validated change in dietary behavior in the intervention group.
To validate changes in intraocular pressure (IOP) after phacoemulsification with intraocular lens (IOL) implantation without pressure-lowering surgery in previously unoperated eyes of normal and glaucoma patients.
To examine and validate changes in striatal dopaminergic function and regional brain metabolism during phenocoversion of preclinical Huntington's disease (pHD).
Quantitative-PCR (qRT-PCR) was used to validate changes in expression levels obtained from DNA-macroarray experiments (Table 2).
