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These in silico predictions were then validated by quantifying the levels of miRNAs and target mRNAs by RT-PCR.
The ability of OPS imaging to analyse tumour angiogenesis was validated by quantifying the functional vessel density, defined as the length of red blood cell perfused capillaries per observation area.
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Substrates were dried for 24 h at RT. Surface modification was validated by quantifying changes in contact angle using the sessile drop method on a goniometer (First Ten Angstroms FTA1000, V2.1), and by X-ray photoelectron spectroscopy of key samples (see Supplementary Methods).
The simulator is validated by quantifying its ability to model actual deformations in a set of patients affected by an acute myocardial infarction.
The method was validated by quantifying common sediment physical properties (mass, water content) of samples of different thicknesses (from 250 μm to 2 mm) collected from different sampling sites (mud and sandy-mud sediments) by operators with different levels of training.
The following protocol was validated by quantifying miRNA abundance in C57 (wild-type) and dystrophin-deficient (mdx) mice.
The 135 k Brassica Exon Array was validated by quantifying transcriptome differences between leaf and root tissue from a reference Brassica rapa line (R-o-18), and categorisation by Gene Ontologies (GO) based on gene orthology with Arabidopsis thaliana.
This assay was validated by quantifying fluorescence in feeding-defective C. elegans (eat mutants), and by exposing wild-type nematodes to the neuroactive compounds, serotonin and arecoline.
MSA computation methods are generally validated by quantifying their ability to accurately reproduce benchmark MSAs known to be of high quality.
The primary objective of this study was to propose (and validate) a revised PACS success model from the socio-technical perspective by quantifying the subjective perception of RTs.
We have validated these infection-specific expression differences by using microarrays on a larger set of samples, and by quantifying the expression of individual genes using RT-PCR.
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