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Concentrations of 31 cytokines, chemokines and growth factors were measured before and after 3 cycles (9 weeks) of induction TPE using multi-analyte immunobead-based profiling (Luminex Corp., Austin, TX), with selected analytes validated by a single analyte enzyme-linked immunosorbent assay.
Wagenvoord et al. have outlined the limitations of mathematical models of complex reaction networks validated by a single analyte [ 80].
The outer three-micrometer sections were stained with haematoxylin and eosin and validated by a single pathologist to confirm the presence of cancer and the initial histological diagnosis (AL).
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Whether this represents true continued podocyte loss over the intervening time period is uncertain, and would need to be validated by a larger single trial in which all animals were made diabetic at the same time followed by assessment of podocyte loss in samples at regular intervals.
The mechanical properties of normal weight concrete simulated by the modified model were validated by a series of single finite element numerical tests, including unconfined uniaxial compression, triaxial tension and dynamic tension tests.
The accuracy of Transient model is validated by using a single module to compare the simulation temperature results with experimental measurements, with a maximum time-averaged temperature prediction error of 1.5 °C.
All samples were analysed in triplicate and the specificity of each primer pair was validated by a dissociation curve (a single peak was observed for each primer pair).
The specificity of each primer pair was validated by a dissociation curve (a single peak was observed for each primer pair) (Additional file 2).
The new model is validated by a series of simulations including the single particle settling and well-known 'Drafting, Kissing and Tumbling' (DKT) phenomenon found in suspensions.
However, these results are not validated by a re-sampling technique as bootstrapping or cross-validation and are based only on a single marker.
The results for the single channel case have also been validated by a comprehensive computational model whose formulation is described.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com