Sentence examples for validated assembly from inspiring English sources
A revised, error-corrected, and validated assembly of the Nipponbare cultivar of rice was generated using optical map data, re-sequencing data, and manual curation that will facilitate on-going and future research in rice.
The validated assembly contained six Sp185/333 genes that were clustered in a 34 kb region at one end of the BAC with five of the six genes tightly clustered within 20 kb.
Both the sequence and optical mapping data were generated as part of a larger project that produced a 'revised, error-corrected, and validated assembly of the Nipponbare cultivar of rice' (Kawahara et al. 2013).
Tolerance analysis is an important step to validate assembly process planning scenario.
Completeness was confirmed by validating assembly graph connectivity, and also by considering expected genome features such as single copy genes.
Sequence data from the two OsBRI1-containng BAC representatives not only allow for comparative genomic analysis of the switchgrass genome but also provide references to validate assembly of contigs and evaluate switchgrass genome using additional molecular approaches.
In order to validate assembly quality, we annotated the transcriptomes by BLAT comparisons to 10,602 honeybee reference protein sequences that are not members of the focal chemosensory gene families (non-chemosensory (NC) gene set; RefSeq database accessed 10/22/12).
We obtained draft contig data of GGS_124 from a commercial service (454 Life Sciences, Branford, CT), and the gaps between the contigs were tiled by PCR after closure to validate assembly using specific primers or by primer walking and an ABI 3100 genetic analyzer (Applied Biosystems Inc., Foster City, CA).
If the Fosmids are to be used to help validate assemblies, only those regions that we believe to be accurately assembled should be utilized.
Finally, they validate assemblies based solely on BAC fingerprints.
This emphasises that in all cases in which the exact order of sequence contigs on chromosomes needs to be known it may be necessary to validate assemblies.
