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We observed this effect when miRNAs already validated as true regulators of VEGF were co-transfected with an miRNA validated as a false positive regulator.
We hypothetically state that SVs meeting the abundance constraint (span-size deviation) × n >500 can be validated as true deletions.
While a few non A-to-Gs (noncanonical editing) sites might be expected, none could be validated as true editing sites.
In a recent study, over 80% of the SNPs derived from GenBank sequences have been validated as true SNPs in the eastern oyster [ 35].
Preliminary hits from the profiler screen (<40% kinase activity remaining) were validated as true positives or false hits by a follow-up determination of an IC50 value.
We chose these two algorithms because most of the randomly selected miRNA targets predicted by mirTarget2 and PicTar have been validated as true targets [ 50, 52].
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For these 43 WES samples, 118 putative sSNVs were validated as true-positives.
This is not always true, as some sites within dbSNP have validated as true-positive somatic mutations in cancer.
In only one case was the PET intra-abdominal nodal result unable to be validated as a true negative, as the patient died within 12 months of follow-up of unrelated causes.
However, only a few of these candidates have been validated as being true adipokines (time dependent secretion) and the regulation or function is unknown for most of the potential adipokines [ 10].
The anti-proliferative effect of CCM+DHA on SK-BR-3 cells is validated as a true synergy by molecular level characterization indicating that the emergent cell signaling network state is unique to the combined use of the two compounds.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com