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These amplicons contained 225 SNPs that had been validated in our gold standard deep whole genome call set (see methods), which includes 43 previously validated as high-confidence SNPs from Hap Map 3.3.
Tumour and normal mucosa morphology on the arrays were validated as having high accordance with that of the whole archived section.
DASH was also validated as a high-throughput method for characterisation and classification of enzymes based on their products, here shown for different xylanases.
The evaluation of trabecular bone by μCT was validated as an alternative technique with a high correlation between μCT and histology based morphometry.
Our study demonstrates that the top six most stably expressed miRNAs (let-7a, miR-16, miR-26a, miR-345, miR-425 and miR-454) described herein should be validated as suitable reference genes in both high-throughput and lower throughput RT-qPCR colorectal miRNA studies.
The suggested methods have high sensitivity and are validated as per ICH guidelines and rely on the use of non-expensive technique and affordable easily available chemicals.
The cumulative score of these 17 groups has been validated as a prognostic indicator in large patient groups, with higher scores indicating a higher risk of dying.
However, at the moment, a high number miRNAs have been validated as specific for sepsis.
EPCs have been validated as CV risk biomarkers for both low- and high-risk population [ 158– 158].
Furthermore, high miR-21, high miR-31, and low miR-375 tumoral expressions have been validated as independent prognostic biomarkers for poor overall survival in PDAC.
A remarkably large number of genes already validated as altering behavioral responses to ethanol were contained in the higher ranks.
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