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Finally, we show that selected predicted target genes can be validated as functional targets of specific microRNAs in vitro.
9 miRNAs have been previously validated as functional activators or inhibitors of EMT or MET.
Neither His (H), Gln (Q), nor Arg (R) had been previously validated as functional PTS1 tripeptide residues at pos. -3.
The peptide sequences, however, can be validated as functional inhibitors upon expression in cells or by exogenous transduction into cells mediated by protein transduction domains.
On the other hand, two predicted non-canonical PTS1 tripeptides could not be validated as functional PTS1 tripeptides for the chosen Arabidopsis sequences, namely those terminating with HKL > and RKM>.
Although a small set of TFBSs has been experimentally validated as functional and recorded in reference databases, this probably represents an insignificant portion of the entire set of TFBSs in the human genome.
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Moreover, by using the well known OLSR protocol, which is a standard proactive protocol from the IETF MANET working group, the CGP protocol can be validated as a functional protocol for MANETs.
18F-F DGal PET/CT has been validated as a functional measure in pigs [10], healthy human subjects [11], and patients with cirrhosis [4].
Thus, the variant was validated as a functional single nucleotide polymorphism.
The QRL > decapeptide validated as a functional PTS1 domain derived from the second alternative splice variant of a DNAJ homolog.
One short TA module has been validated as a functional TA pair in S t. aureus (Fu et al. 2007).
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