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i) GP as 'patient advocate' GPs discussed how work assessments validated absence from the workplace and highlighted the potential tension between their gatekeeper and patient advocacy roles.
We addressed the role of SOX17 during hPGCLC specification by generating SOX17 KO NANOS3-mCherry hESC line and validated absence of SOX17 expression in day 4 embryoids from mutant cells by western blot and immunofluorescence.
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The same type of experiment was performed with BIP2 to validate absence of BiFC between AbdA and BIP2.
Selected optimum conditions were also validated against absence of crude glycerol (4.69 ± 0.76), with pretreated crude glycerol (20.06 ± 0.51) and across mono-culture system (15.43 ± 0.79 to 22.14 ± 0.94).
A number of controls validated the absence of significant quantities of residual genomic DNA.
Melt curve analysis, agarose gel electrophoresis, and DNA sequencing validated the absence of illegitimate cross-amplification of other paralogues.
This validated the absence of significant plate-to-plate bias and specifically confirmed longer telomeres in centenarians and (semi- supercentenarians than expected from the regresemi- supercentenarianser ages.
We validated the absence of POUV activity in PVD2 using a synthetic POUV-responsive reporter gene and assessed the specificity of the new Xlpou25 MO by in vitro translation and rescue experiments.
Their absence in DRM fractions validates the absence of contamination by intracellular membranes (Figure 2A, a).
General shortcomings of the included articles were the absence of a validated 'information' questionnaire, absence of information about the degree of selection of the patient sample, a cross-sectional design, and the lack of comparison between two groups or time points.
The document mentions only one check carried out by the NSA on the raw intelligence, saying the agency will "regularly review a sample of files transferred to ISNU to validate the absence of US persons' identities".
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com