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We used the tools validated above to accurately estimate the proportion of MSNs in the striatum, taking into account both DARPP-32 immunoreactivity and nuclear morphology.
Considering the repressive role of the AGO1-enriched sRNAs in gene expression, we deduced that for the sRNA target pairs validated above, the target genes should display expression patterns contrary to those of the sRNA regulators.
We have validated above the multi-watermark detector using a static function, (mathcal {I}), to define the multi-watermark and different performance loss between single- and multi-watermarks.
Abundance levels of tiGHR I mRNA (target) in different tilapia tissues were measured using the quantitative RT-PCR by competition validated above.
The serum was diluted ten-fold with dilution reagent to measure C- terminal FGF23, since the assay accuracy was not validated above this dilution level.
The NCK1 gene, described and validated above (Fig. 2B), have two promoters; the upstream P1 at the canonical promoter and P2, which is an alternative TSS and preferentially used after TNF-α treatment.
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Further site-directed mutagenesis analyses demonstrated that the FadR mutants (F74G and/or W75G) do not exhibit the detected DNA-binding activity, validating above structural reasoning.
To validate above results, the corresponding proteins were detected again by Western blots in these monoclonal cell lines.
They validated the above equation with real field injection data.
Application of universal kriging with the anisotropic monomial model for the June 19th , 1988hailstorm validated the above results.
The developed finite element method described and validated in above is used to design a deep beam, whose dimensions are shown in Fig. 5.
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