The IHC results further validated the higher expression of these two chemokines in the AS group than the OA group.
Here we designed a pilot study to validate a high-throughput assay to measure the expression levels of key regulators of kidney development in single cells isolated from embryonic mice.
In addition, qRT validated higher expression levels for Mo17 even at 300 μM iron but also suggested a further significant induction at 10 μM iron for Mo17.
On the other hand, the lower representation of de novo FA synthesis observed in the H group was validated by the higher expression of the thyroid hormone responsive (THRSP) gene (fold difference = 3.46, p-value = 3.36 × 10-06).
To validate the presence of lymphocytes in those samples that show a high expression of the respective metagenes, we performed immunohistochemistry using specific antibodies.
We performed quantitative RT-PCR to validate higher expression of nkx2.2 and olig2 in the zebrafish SRBCTs (Fig. 3C).
The suitability of safflower KASII gene as an internal reference gene was validated by its high expression stability in various tissues and developmental stages (unpublished data).
Indeed, candidate genes were validated in a high yield.
In cohort II, we were able to validate that high tumour expression of STMN1 is correlated to higher risk of death in cancer within 2 years in T2 T4 patients.
Similarly, Hu and colleagues [ 11] recently described and validated a refined 'intrinsic' classification signature that is conserved across microarray platforms and also uncovered a possible 'new' subtype characterized by the high expression of interferon-regulated genes.
