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The purpose of the CSP-CIPer was initially to genotype HPV plasmids -16 and -18, which produced amplicons visualized as agarose gel-bands at the expected size range of 185-188 base pairs and correctly validated with Pyrosequencing (Figure S2).
The hybridization of fluorescently-labelled unamplified ChIP DNAs, coupled with a highly sensitive and quantitative array platform [19], allowed us to identify reproducibly (Figure S1), and validate with real-time PCR (Figure S2), a wide range of regulatory features across the SCL locus as described below.
Technically, the microarray gene level expression estimates made by MMBGX appear robust and, if anything, highlight inadequacies in the qPCR assays used to validate them (Figures 2 and S4), with the failure to detect hTERT mRNA in wtBAC-LCLs the only exception.
Forest production statistics are collected by direct assessment in the field, with other methods being used to attempt to validate the figures.
We firstly validated that the TaLE framework solves both problems of unfairness and service differentiation that we dealt with at Section 1 with Figures 1-2.
Fixed cells were stained for F-actin with phalloidin or Cofilin1 using the antibody validated in Figure 6.
These results are validated by Figure 6a,b.
These findings, shown in Figure 4, were validated with considering hub genes in three centrality contexts: degree, betweenness and closeness.
These findings were validated with qRT-PCR using the validation cohort (N = 32) (Figure 2A and 2B).
This model is validated with a Finite Element Method (FEM) analysis shown in Figure 7. Figure 7 FEM analysis of the semi-open climate chamber.
Protein expression was validated with qPCR and Western blot displayed in Figure 5.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com