We first validated the utilization of these techniques by evaluating the behavior of Akt1, which has been previously and extensively addressed [22].
The confirmation of compound activity in the age dependent Htn-Q150 model validated the utilization of the pqe-1 sensitized strain as a rapid means of identifying HD therapeutic compounds.
As expected, the western blot analysis of the immunoprecipitates indicated that EGFP-AGS3, but not EGFP alone, co-immunoprecipitated with Gαi3 (the bottom panel, Fig. 1A), thus validating the utilization of EGFP-AGS3 to pull down AGS3-associated proteins.
A previous study [19] validated the utilization of "massively parallel signature sequencing" (MPSS) [20] to analyze transcriptional regulation in a closely related dinoflagellate (Alexandrium fundyense) and provided evidence for the complexity of the transcriptome, the presence of gene families, and the extent of transcriptional regulation.
Having obtained sufficient evidence from stringent transcript alignments, we pursued to validate the functional utilization of tandem splice sites from independent lines of evidence.
A health research system is defined as the people, institutions, networks and activities whose primary purpose is to generate, communicate and promote the utilization of scientifically validated knowledge that can be used to enhance, restore and/or maintain the health status of populations [ 23].
The utilization of this conditioning procedure was validated by determination of Pb2+ concentrations down to ca 20 ppb in aqueous samples by Pb2+-ISEs and by comparing the results with those obtained by ICP-MS.
The utilization of the new model is validated through extensive simulation studies under different conditions that are faced in practice.
The utilization of a thin try cut needle has been validated in a previous publication [ 22].
