To validate the reverse-signaling hypothesis, a Transwell migration assay was performed by treating NOBEC expressing ecto-ErbB4 and wild type (WT) NOBEC with DAPT, a specific inhibitor of γ-secretase, the enzyme which mediates NRG1 proteolytic cleavage, releasing a cytoplasmic fragment.
Microarray results of selected genes were validated using the reverse transcriptase-multiplex ligation dependent probe amplification (RT-MLPA) method [ 65].
Microarray expression of differentially accumulated transcripts identified in the Flynn trial in autumn (Flynn-LW) were validated using the reverse transcriptase-multiplex ligation dependent probe amplification (RT-MLPA) method [ 67].
Four types of experiments were performed to validate the Standard, Reversed and Sham TMS delivered from the Fig8 and Circ coils.
In order to validate the collection for reverse genetics, DNA pools were screened for two genes coding enzymes of the lignin biosynthesis pathway: Coumarate-3-Hydroxylase (C3H) and Cinnamyl Alcohol Dehydrogenase (CAD).
To validate the finding, we reversed the analysis and compared each non-autism control to a single mean value for each miRNA across all autism cases.
Subjects are assumed to continue to acquire information following the response, which would allow them to validate or reverse the response.
To further validate the HTTS data, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed.
To further validate the microarray data, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed.
The expression patterns of 20 differentially expressed genes were further investigated to validate the SSH data by reverse transcription PCR (RT-PCR) and real-time PCR analysis.
To validate the results obtained by reverse northern analysis, RNA accumulation of ten ESTs (FL512354, FL512338, FL512352, CD051280, FL512397, CD051326, CD051266, FL512439, FL512463 and FL518919) was monitored in both the cultivars by northern analysis.
