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Following their application, functional genomic analyses are necessary to validate the function of any candidate sequences or alleles identified by the phylogenetic footprinting and shadowing methods; but even by themselves, both methods can provide valuable insights into the location of potential cis-regulatory sequences and the transcription factors that bind them.
Some functional genomics approaches like qRT-PCR may validate the function of these genes (Hu et al. 2009).
These results confirmed the functional incompatibility types of the S-haplotypes previously identified as S01−S08 and validate the function of four other putative S-haplotypes S09 (A06/ A07; Figure S9), S10 (B05; Figure S10), S12 (B17/ B18; Figure S12) and S13 (A04; Figure S13), but not S11 (B09/ B12; Figure S11).
Significant experiment has been designed to validate the function of the device.
The HIL system emulates battery cell dynamics to validate the function of voltage monitoring.
Some of these parasite proteins are undergoing RNA interference or knockout analyses to validate the function of their corresponding genes.
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By expressing 4-OH-ICN biosynthetic enzymes in Saccharomyces cerevisiae and Nicotiana benthamiana, we reconstitute the complete pathway in vitro and in vivo and validate the functions of its enzymes.
The test track is driven six times by expert drivers to validate the functions of ViDCo.
Recently, a growing body of literature (Table S8) has made it possible to validate the functions of PRdmiRs and RdmiRs, many of which were found to be expressed in tumors.
Future gene function experiments including over-expression and gene silencing are necessary to validate the functions of these LSGs.
However, a high-throughput method will eventually be necessary to experimentally validate the functions of sequenced genes.
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