Sentence examples for validate the expression changes from inspiring English sources

To validate the expression changes found in Microarray Experiment 1, transcript levels of 108 genes and three reference genes were quantified by Q-PCR (with validated primers [ 10]).

To validate the expression changes of ESR1 after DAC treatment, two publically available expression datasets were mined for data regarding DAC treatment of two breast cancer cell lines used in this current study (gse10613 and gse13733) [ 23, 24].

To validate the expression changes found in the microarray experiments, transcript levels of the ten selected genes were quantified by the ABI Prism 7700 (Applied Biosystems, Foster City, CA, U.S.A) as described by Mascarell-Creus and col.

To validate the expression changes identified in the microarray, TaqMan qPCR analysis was conducted on select groups of genes involved in metabolism using cDNA isolated from WT and KO E17.5, neonates and Snrk knockdown CM (Fig. 4B D).

To validate the expression changes found in the microarray experiments, transcript levels of the 12 selected genes transcripts were quantified by the ABI Prism 7700 (Applied Biosystems, Foster City, CA, U.S.A).

To validate the expression change of candidate genes, qRT-PCR assays were used.

The main criteria applied for the final selection of the pathways were: 1. the last step of the pathway has to indicate molecular synthesis/expression so as to validate the expression change described on the microarray result, 2. confirmation of the annotation of the selected genes by sequencing and 3. validation of the literature references used by the program.

Real-time quantitative PCR validated the expression changes of 12 selected genes.

I validated the expression changes of some representative target genes by quantitative PCR (Q-PCR).

In the present study we validated the expression change of four specific transcripts by real-time RT-PCR.

We then validated the expression change of these four miRNAs by real-time PCR on both regions of the monkey brains and in the human brain, and confirmed significant upregulation of three: miR-21, miR-142-3p miR-142-3p miR-142-3p