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Additional characterization of their binding elements through mutation analyses and reporter assays should further validate the assignment.
This variable was then used as an external indicator to validate the assignment of samples to the three clusters.
In order to validate the assignment of individuals with apparently no spores attached to their oesophagus, we viewed the oesophagus of slightly squashed animals at 400 × magnification.
Consensus decision making exploits the goodness of multiple search algorithms to validate the assignment results of spectral data at a relatively low cost.
The band assignments were made using the asynchronous plot for which the highest spectral resolution was obtained and extrapolated to the synchronous and baseline-corrected spectral overlay for each data set to validate the assignment.
To validate the assignment of BACs to the two subgenomes, PCR products derived from at least two BACs each ascribed to the A and C subgenomes, respectively were subjected to sequence analysis, if available.
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This further validates the assignment of the glycosylation site.
Immunoblotting also validated the assignment of some of these proteins.
The gold clusters clearly pinpointed the C-termini of the Med17 and Med22 subunits at roughly the same position on the upper-right portion of the core Mediator, which directly validates the assignment of the Head module.
Although we have not experimentally validated the assignment of snoRNA genes to UTRs, these assignments are likely to be correct since we found that snoRNA genes overlapped with protein-coding region of the gene as well as the UTR.
Although the functions of the proteins/unigenes still need to be further validated, the assignment of functions provides important reference for identification of the targets in the in vivo experiment.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com