To validate the radiation sensitization by RK-33, we generated an orthotopic lung cancer model by injecting A549 cells into the tail vein of athymic NCr-nu/nu mice.
Also, although the validated ISAAC questionnaire was used to identify children with allergic rhinoconjunctivitis, such identification by parents was prone to subjectivity (e.g., attributing common cold to allergic rhinoconjunctivitis or vice versa), and we did not have data on objective tests (e.g., pollen sensitization) to validate parental reports for this outcome.
The function of dLmo has been validated in mammals, as downregulation of one of the mammalian homologs, Lmo4, causes increased cocaine sensitivity and sensitization in mice (Lasek et al. 2010).
Validated tools to predict respiratory sensitization and to translate irritancy assays to asthma potency are needed, in addition to diagnostic biomarkers that assess and differentiate allergy versus irritant-based asthmatic responses.
To develop and validate a prediction model to detect sensitization to wheat allergens in bakery workers.
Since an accurate diagnosis dictates the administration of anti-IgE therapy, further research is needed to validate BALF IgE levels in fungal sensitization.
Our results of cisplatin sensitization from NSCLC were however validated also in ovarian cancer cells which were sensitized to cisplatin upon siRNA knockdown of DKK1.
In order to validate the accuracy of the computational results, radiotherapy sensitization of Rhein to NPC cells and its effects on the expression of target proteins were evaluated separately by CCK8 assay and Western blotting analysis.
Furthermore, we used previously validated outcomes with relevance to asthma or allergic sensitization.
The fact that sensitization can occur in locations distant from the sensitizing stimulus (duodenum) suggests a central site of sensitization.
The goals of this study were to: (i) compile, curate, and integrate the largest publicly available dataset of chemicals studied for their skin permeability; (ii) develop and rigorously validate QSAR models to predict skin permeability; and (iii) explore the complex relationships between skin sensitization and skin permeability.
