Sentence examples for validate reporter from inspiring English sources
To validate reporter expression with endogenous LepRb, we mapped and quantified colocalization of tdTOM-IR and EYFP-IR with leptin-induced pSTAT-IR.
This protocol provides in-depth, detailed steps to generate and validate reporter cells to measure autophagic flux, identify and properly set up an adequately equipped flow cytometer, and use it to quantify and sort cells based on their relative levels of autophagic flux (Fig. 1B).
Figures 1 and 2 show flowcharts of the process used to validate reporters by placement on the reference assembly.
For measurement of cell engraftment after 24 h, a previously validated reporter gene based approach was utilized in eight rats [ 26].
Therefore, in this study we have developed and validated reporters designed to partially reduce this complexity and facilitate the analysis of N-glycosylation in C. albicans.
Figure 1 shows a flowchart of the process used to validate a reporter through alignment to RefSeq RNAs.
To validate the reporter cell line, we established stable knockdown lines using shRNAs directed against Eed and Ring1B, core components of PRC2 and PRC1, respectively.
To validate our reporter gene assay and rule out any technical differences in luciferase activity in NALM6 vs. CCRF-CEM cells, we assayed a CMV promoter-luciferase driven vector (pCMV-luc) and found no significant differences in both cell lines (data not shown).
We define methods for validating the reporter constructs and describe how they can be used to generate stable transgenic lines in Xenopus.
Validating this reporter system in our hands, we first showed that Rapamycin treated, mRFP-GFP-LC3-expressing cells which underwent complete autophagic flux indeed exhibited predominant red fluorescence with only dim green fluorescence (Fig. 5C).
We validated the reporter in vitro and demonstrated that its activity could be differentially modulated by EGFR tyrosine kinase inhibition with erlotonib or receptor activation with epidermal growth factor.
