However, PCR did not validate regulation of transforming acidic coiled-coil containing protein 2 and coiled-coil containing
The experimental version only contains experimentally validated regulations whereas the predicted version additionally contains the transferred evolutionarily conserved regulations as well.
Especially, EpimiR modeled all experimentally validated regulations as the miR2Epi pathway or Epi2miR pathway, which presents components of the pathway in bold in the 'Detail' page (Table 1).
Databases, such as TransFAC [ 2] on TF-to-mRNA regulation, TransmiR [ 3] on TF-to-miRNA regulation, and TarBase [ 4] on miRNA-to-mRNA regulation, provide experimentally validated regulation relationships between regulators and their targets.
b) Inferring regulatory interactions from literature: GRNs of differentially expressed gene sets were reconstructed using experimentally validated regulation information from literature.
Finally, by resorting to databases TarBase, TransFAC, and TransmiR which contain different types of experimentally validated regulation relationships, we compared the fractions of experimentally supported records in the modeling results and in the forward predictions.
We have performed follow-up experiments that validate conditional regulation by two FFRPs, and demonstrate a context dependent fitness benefit for the regulation.
In 2010, in Comcast v. the F.C.C., the same appeals court rejected the legal theory that the agency was using to validate its regulation of broadband Internet service.
Quantitative real time PCR, Western blotting, and luciferase reporter assay were carried out to validate genes regulation and targeting by miRNAs.
To validate the regulation β-catenin by JNK2 in vivo, JNK2-/ and +/+ mice were dissected.
Due to rarity of CIS specimens, we were not able to validate up-regulation of gene expression directly.
